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earray – custom microarray design application  (Agilent technologies)


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    Agilent technologies earray – custom microarray design application
    Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species <t>microarray</t> platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.
    Earray – Custom Microarray Design Application, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/earray – custom microarray design application/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    earray – custom microarray design application - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray"

    Article Title: Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0075553

    Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species microarray platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.
    Figure Legend Snippet: Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species microarray platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.

    Techniques Used: Microarray, Transformation Assay, Expressing, Standard Deviation

    The mRNA level fold changes observed between exposed and control fish are depicted as a diagnostic heat map representative of all the genes present on the multi-species array. The log 2 ratio for each gene is defined as the mean of all representations of that gene on the microarray; these include probes from different regions of the gene, as well as from different species of fish (individual log 2 ratios for each unique probe are calculated as the median of 46 replicates present on the array). The range of colors is between -8-fold and +8-fold and preserves qualitative relationships among individual values. All fold changes outside of this range have been truncated to ± 8.
    Figure Legend Snippet: The mRNA level fold changes observed between exposed and control fish are depicted as a diagnostic heat map representative of all the genes present on the multi-species array. The log 2 ratio for each gene is defined as the mean of all representations of that gene on the microarray; these include probes from different regions of the gene, as well as from different species of fish (individual log 2 ratios for each unique probe are calculated as the median of 46 replicates present on the array). The range of colors is between -8-fold and +8-fold and preserves qualitative relationships among individual values. All fold changes outside of this range have been truncated to ± 8.

    Techniques Used: Diagnostic Assay, Microarray

    Multi-species SYBR green Q-PCR validation of multi-species microarray for (A) CYP3A (B) Vit1 (C) Vit2 (D) ESR1/ ERα (E) ESR2/ ERβ specific transcripts. GAPDH -normalized fold changes (based on triplicate measurements) of gene expression in turbot liver from selected impacted sites with respect to reference fish are presented. Each fold change was derived from the mean log 2 ratio between each fish and a reference derived from two control fish. Vit1 and Vit2 transcripts were strongly up-regulated in all fish. ERα was down-regulated in one fish and up-regulated in two others relative to control fish. ERβ was down-regulated in all fish examined relative to control fish. CYP3A was up-regulated in eight and down-regulated in two fish.
    Figure Legend Snippet: Multi-species SYBR green Q-PCR validation of multi-species microarray for (A) CYP3A (B) Vit1 (C) Vit2 (D) ESR1/ ERα (E) ESR2/ ERβ specific transcripts. GAPDH -normalized fold changes (based on triplicate measurements) of gene expression in turbot liver from selected impacted sites with respect to reference fish are presented. Each fold change was derived from the mean log 2 ratio between each fish and a reference derived from two control fish. Vit1 and Vit2 transcripts were strongly up-regulated in all fish. ERα was down-regulated in one fish and up-regulated in two others relative to control fish. ERβ was down-regulated in all fish examined relative to control fish. CYP3A was up-regulated in eight and down-regulated in two fish.

    Techniques Used: SYBR Green Assay, Microarray, Expressing, Derivative Assay



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    earray – custom microarray design application  (Agilent technologies)
    90
    Agilent technologies earray – custom microarray design application
    Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species <t>microarray</t> platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.
    Earray – Custom Microarray Design Application, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/earray – custom microarray design application/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    earray – custom microarray design application - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species microarray platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.

    Journal: PLoS ONE

    Article Title: Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray

    doi: 10.1371/journal.pone.0075553

    Figure Lengend Snippet: Comparison of the performance characteristics of on both prototype (spotted oligonucleotide) and second generation (Agilent ink jet oligonucleotide) endocrine multi-species microarray platforms; Panels A and B: scatter plots of log 2 signal intensities of an individual fish from the Los Angeles Sanitation District versus the control fish pool. Panels A and B depict prototype and second generation platforms respectively. Panel C; direct comparison of platform sensitivity defined as log 2 ratio between treatment and ABC control. Panel D: Analysis of signal concordance. Panel E: Analysis of variance with both platforms. To measure internal consistency (variance of internal replicates), log 2 transformed expression value was plotted on the x-axis. Standard deviation is plotted on the y-axis, with a range of plus or minus one standard deviation from the mean expression value. The y-axis is a measure of the deviation of individual replicate probes stray from the mean value. The second generation platform has smaller variance of the internal replicates. Probe variance on both platforms is independent of the signal. Panel F: Comparison of the probe signal intensity distribution (plotted as log 2 transformed) for 149 probes that were present on both platforms.

    Article Snippet: We designed in silico a 60-mer oligonucleotide-based multi-species microarray using the eArray – Custom Microarray Design Application from Agilent (Santa Clara, CA).

    Techniques: Microarray, Transformation Assay, Expressing, Standard Deviation

    The mRNA level fold changes observed between exposed and control fish are depicted as a diagnostic heat map representative of all the genes present on the multi-species array. The log 2 ratio for each gene is defined as the mean of all representations of that gene on the microarray; these include probes from different regions of the gene, as well as from different species of fish (individual log 2 ratios for each unique probe are calculated as the median of 46 replicates present on the array). The range of colors is between -8-fold and +8-fold and preserves qualitative relationships among individual values. All fold changes outside of this range have been truncated to ± 8.

    Journal: PLoS ONE

    Article Title: Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray

    doi: 10.1371/journal.pone.0075553

    Figure Lengend Snippet: The mRNA level fold changes observed between exposed and control fish are depicted as a diagnostic heat map representative of all the genes present on the multi-species array. The log 2 ratio for each gene is defined as the mean of all representations of that gene on the microarray; these include probes from different regions of the gene, as well as from different species of fish (individual log 2 ratios for each unique probe are calculated as the median of 46 replicates present on the array). The range of colors is between -8-fold and +8-fold and preserves qualitative relationships among individual values. All fold changes outside of this range have been truncated to ± 8.

    Article Snippet: We designed in silico a 60-mer oligonucleotide-based multi-species microarray using the eArray – Custom Microarray Design Application from Agilent (Santa Clara, CA).

    Techniques: Diagnostic Assay, Microarray

    Multi-species SYBR green Q-PCR validation of multi-species microarray for (A) CYP3A (B) Vit1 (C) Vit2 (D) ESR1/ ERα (E) ESR2/ ERβ specific transcripts. GAPDH -normalized fold changes (based on triplicate measurements) of gene expression in turbot liver from selected impacted sites with respect to reference fish are presented. Each fold change was derived from the mean log 2 ratio between each fish and a reference derived from two control fish. Vit1 and Vit2 transcripts were strongly up-regulated in all fish. ERα was down-regulated in one fish and up-regulated in two others relative to control fish. ERβ was down-regulated in all fish examined relative to control fish. CYP3A was up-regulated in eight and down-regulated in two fish.

    Journal: PLoS ONE

    Article Title: Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray

    doi: 10.1371/journal.pone.0075553

    Figure Lengend Snippet: Multi-species SYBR green Q-PCR validation of multi-species microarray for (A) CYP3A (B) Vit1 (C) Vit2 (D) ESR1/ ERα (E) ESR2/ ERβ specific transcripts. GAPDH -normalized fold changes (based on triplicate measurements) of gene expression in turbot liver from selected impacted sites with respect to reference fish are presented. Each fold change was derived from the mean log 2 ratio between each fish and a reference derived from two control fish. Vit1 and Vit2 transcripts were strongly up-regulated in all fish. ERα was down-regulated in one fish and up-regulated in two others relative to control fish. ERβ was down-regulated in all fish examined relative to control fish. CYP3A was up-regulated in eight and down-regulated in two fish.

    Article Snippet: We designed in silico a 60-mer oligonucleotide-based multi-species microarray using the eArray – Custom Microarray Design Application from Agilent (Santa Clara, CA).

    Techniques: SYBR Green Assay, Microarray, Expressing, Derivative Assay